The mezzo-soprano Štěpánka Pučálková graduated at the University Mozarteum in Salzburg, Austria. There she worked under the guidance of Univ. Prof. Elisabeth Wilke KS and Univ. Prof. Eike Gramss. That same year, she was awarded by the organization Stiftung the Mozarteum Medal of Lilli Lehmann. She performed in many productions at the Salzburg Mozarteum University and the Landestheater in Salzburg, performing such roles as Mozart´s Idamante (Idomeneo), the 2nd Lady (Die Zauberflöte) and Marcellina (Le nozze di Figaro), Octavian (R. Strauss: Der Rosenkavalier), Larina (Tchaikovsky: Eugene Onegin), Galatea (Händel: Aci, Galatea et Polifemo) and Annina (Verdi: La traviata). In summer 2013, she performed as Lehrbube at the Salzburger Festspiele (Wagner: Die Meistersinger von Nürnberg) with the conductor Daniele Gatti and director Stefan Herheim. In March 2014, Štěpánka performed the role of Sesto (Mozart: La clemenza di Tito) in Salzburg and at the Teatro Romano Fiesole in Florence (Italy). In autumn 2014, she made her debut at the State Opera in Prague as the Servant (R. Strauss: Salome). Štěpánka has also performed as Suzuki (Madama Butterfly), Stéphano (Roméo et Juliette), 3rd Lady (Mozart: Die Zauberföte), and 1st Maidservant (R. Strauss: Elektra). At the National Theatre Opera in Prague she has performed as Charlotte (Massenet: Werther). She has sung at the Slovak National Theatre in Bratislava (Rosina in Rossini's Il barbiere di Siviglia and Annio in La clemenza di Tito), as well as Mercedes (Bizet: Carmen) in Landestheater Bregenz und Landesthetare Salzburg. She also made her debut as a guest at the Tiroler Landestheater Innsbruck as Kitchen boy (Dvořák: Rusalka). She received 2nd Prize as The Best Female Voice during the Concours international de Belcanto Vincenzo Bellini Competition in Marseille, France. During the 2016/17 season, she made her debut in the role of Siegrune (R. Wagner: Die Walküre) during the famous Osterfestspiele Salzburg, conducted by Christian Thielemann. She also travelled to China with this production in cooperation with the Beijing Music Festival with the support of the Hong Kong Philharmonic Orchestra. Next year under the direction of Rolando Villazón, she will perform as a soloist in the Mozart′s Davide penitente during the Mozartwoche festival in Salzburg. Since the season 2018/19, she is a member of the Semperoper Dresden Ensemble.